鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究

doi:10.11843/j.issn.0366-6964.2019.01.007

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (1): 52-60.doi: 10.11843/j.issn.0366-6964.2019.01.007

鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究

罗华伦^1,2, 李万贵³, 张依裕^1,2*, 吴磊^1,2, 覃媛钰^1,2

1. 贵州大学动物科学学院, 贵阳 550025;
2. 高原山地动物遗传育种与繁殖教育部重点实验室, 贵阳 550025;
3. 六盘水市草地工作站, 六盘水 553001

收稿日期:2018-04-20 出版日期:2019-01-23 发布日期:2019-01-23
通讯作者: 张依裕,主要从事动物遗传资源评价与利用研究,E-mail:zyy8yyc@163.com
作者简介:罗华伦(1993-),女,布依族,贵州安龙人,硕士生,主要从事动物遗传资源评价、保护与利用研究,E-mail:463809726@qq.com
基金资助:
国家自然科学基金（31160453）

Effect of Duck LXRα Gene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells

LUO Hualun^1,2, LI Wangui³, ZHANG Yiyu^1,2*, WU Lei^1,2, QIN Yuanyu^1,2

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, Guiyang 550025, China;
3. The Grass Workstation of Liupanshui City, Liupanshui 553001, China

Received:2018-04-20 Online:2019-01-23 Published:2019-01-23

摘要/Abstract

摘要：

旨在探究鸭LXRα基因对脂肪代谢的表达调控机制，本试验以pEGFP-N3为载体，构建携带HIS标签的LXRα基因真核过表达载体，并将过表达载体分别转染鸭原代肝细胞及PC3细胞，使用鸭HIS标签试剂盒测定LXRα基因在肝细胞及PC3细胞中的过表达量，分析该基因分别在两种细胞中过表达与各脂质指标含量的相关性，进而探究其对鸭肝细胞及PC3细胞脂质代谢的调控作用。试验结果显示，LXRα基因在两种细胞中过表达与三酰甘油含量（triglyceride，TG）及高密度脂蛋白（high density lipoprotein，HDL）含量均呈极显著正相关关系（P<0.01），与总胆固醇（total cholesterol，TC）含量及低密度脂蛋白含量（low density lipoprotein，LDL）则均呈极显著负相关关系（P<0.01）。本研究揭示了LXRα基因过表达对TG和HDL的合成代谢有重要的正向调控作用，对TC和LDL具有反向调控作用，且在这两种类型的细胞中调控机制相似。这一研究结果将对今后深入研究LXRα基因在脂质代谢网络通路中的作用及解决鸭体脂肪过度沉积等问题奠定理论基础。

Abstract:

To explore the regulation mechanism of duck LXRα gene on fat deposition,in this experiment,the pEGFP-N3 was used as the vector to construct the LXRα gene eukaryotic over-expression vector carried HIS-tag that was transfected into the duck primary hepatocytes and PC3 cells, respectively. The over expression level of LXRα gene in duck hepatocytes and PC3 cells were detected by the duck HIS-tag kit,and the correlation between the over expression of LXRα gene in the two kinds of cells and the lipid index contents was analyzed,and the regulation mechanism of LXRα gene on lipid metabolism in duck hepatocytes and PC3 cells was explored. The results showed that the over expression of LXRα gene in both cells were significantly positively correlated with the contents of triglyceride (TG) and high-density lipoprotein (HDL) (P<0.01),and significantly negatively correlated with the contents of total cholesterol (TC) and low density lipoprotein (LDL) (P<0.01). The results reveal that over expression of LXRα gene has a positive regulatory effect on the anabolism of TG and HDL,and has a negative regulatory effect on TC and LDL,and the regulatory mechanisms in the two types of cells are similar. The results of this study will lay a theoretical foundation for the further study of the role of LXRα gene in the lipid metabolism network pathway and the solve of excessive fat deposition in duck body.

中图分类号:

S834.2

罗华伦, 李万贵, 张依裕, 吴磊, 覃媛钰. 鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究[J]. 畜牧兽医学报, 2019, 50(1): 52-60.

LUO Hualun, LI Wangui, ZHANG Yiyu, WU Lei, QIN Yuanyu. Effect of Duck LXRα Gene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 52-60.

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鸭LXRα基因过表达对原代肝细胞及PC3细胞脂质代谢的效应研究

Effect of Duck LXRα Gene Overexpression on Lipid Metabolism in Primary Hepatocytes and PC3 Cells

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